Everythings seems to work fine with automatic or custom(75%) scaling.
At 100% scaling "Read" "Clear" "Verticle" "Diagonal" and "Statistics" buttons no longer function.
At higher scaling more buttons from the bottom of the UI stop working.
It looks like only the region covered by 75% scaling will take player inputs and anything beyond that will not, but higher scaling will make the UI larger than that region thus placing buttons out of input range. Desktop resolution is 1600x900 (didn't see a resolution option within Factorio?)