This is done with last version of Pinga app, that already can reduce unused palette colours (in PNG optimization script that reduce was made by additional pre-run of pngquant app). There is some minor posibility to reduce filesize of png's some more, but this can be done only with reducing overall number of colours in images that can (and mostly WILL) corrupt visual quality.
P.S. I'm sure that every modder that use .PNG in their mods must optimize their files with PINGA app. This can reduce filesize (up to 90% in some cases). Settings that almost never corrupt quality in our Factorio case are: [Compression = "lossy (strong)"], [Compresion level = "max"], [Quality = right "+" (set to the right position)] , [off dithering]. In many tests this settings provide imo best compression rate without any visible difference ingame. Thanks for listening.