Yeah, sorry, it was the tree seemingly growing up through the tracks. I laid down the tracks using the ghost track planner while holding Shift so it'd mark obstacles for deletion then ran back through with bots to do all of the work. Admittedly I didn't immediately go back through to verify the integrity of the work (the trains ran, so I knew the tracks made a solid connection down through :P), but didn't notice anything out of the ordinary like that until later. Then I noticed several.
I do have Alien Biomes loaded which does change the collision boxes of trees, though. I'll have to pay better attention next time now that I'm aware.